NSJ Bioreagents

MIF Antibody

Product Code:
 
NSJ-R30231
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the MIF antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE rat brain tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE mouse brain tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
3 / 4
Western blot testing 1) human Jurkat, 2) human 293T, 3) rat kidney, 4) rat NRK, 5) rat PC-12, 6) mouse kidney, 7) mouse HBZY-1 and 8) mouse RAW264.7 cell lysate with MIF antibody. Predicted molecular weight ~13 kDa.
4 / 4
Flow cytometry testing of human K562 cells with MIF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIF antibody.
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IHC staining of FFPE rat brain tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
Western blot testing 1) human Jurkat, 2) human 293T, 3) rat kidney, 4) rat NRK, 5) rat PC-12, 6) mouse kidney, 7) mouse HBZY-1 and 8) mouse RAW264.7 cell lysate with MIF antibody. Predicted molecular weight ~13 kDa.
Flow cytometry testing of human K562 cells with MIF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIF antibody.

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CodeSizePrice
NSJ-R30231-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. Localization of the human gene for macrophage migration inhibitory factor(MIF) to chromosome 22q11.2 MIF plays a critical role in inflammatory diseases and atherogenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human MIF (NYYDMNAANVGWNNSTFA) was used as the immunogen for this MIF antibody.
Limitation:
This MIF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14174