NSJ Bioreagents

PD1 Antibody

Product Code:
 
NSJ-R30141
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the PD1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of human 1) Caco-2, 2) SW620, 3) COLO-320, 4) K562, 5) mouse thymus and 6) mouse RAW246.7 lysate. Predicted molecular weight ~32 kda (unmodified), 50-55 kDa (glycosylated).~
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Western blot testing of PD1 antibody and recombinant
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Western blot testing of human 1) Caco-2, 2) SW620, 3) COLO-320, 4) K562, 5) mouse thymus and 6) mouse RAW246.7 lysate. Predicted molecular weight ~32 kda (unmodified), 50-55 kDa (glycosylated).~
Western blot testing of PD1 antibody and recombinant

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NSJ-R30141-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the PD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PDCD1 (Programmed cell death 1), also called PD1, encodes a cell surface receptor that is a member of the B7 superfamily involved in immunomodulation. This gene is mapped to 2q37.3. PDCD1 acts as an inhibitory molecule on T cells after interacting with its ligands PDL1 and PDL2. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. Using flow cytometric analysis, it has been found that expression of PDCD1 was upregulated on CD16-positive and CD16-negative monocytes, but not on dendritic cells, in viremic HIV-positive patients, but not in highly active antiretroviral therapy (HAART)-treated HIV-positive patients. Upregulation in monocytes was induced by microbial Toll-like receptor ligands and inflammatory cytokines.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5133
Immunogen:
Human partial recombinant protein (AA 101-288) was used as the immunogen for this PD1 antibody.
Limitation:
This PD1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse