NSJ Bioreagents

SIP Antibody / SIAH Interacting Protein

Product Code:
 
NSJ-R30852
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution the SIP antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with SIP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human MCF7, 2) human RT4, 3) human SW620, 4) human U-251, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with SIP antibody. Predicted molecular weight ~27 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human HL60 cells with SIP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIP antibody.
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Immunofluorescent staining of FFPE human HeLa cells with SIP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human MCF7, 2) human RT4, 3) human SW620, 4) human U-251, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with SIP antibody. Predicted molecular weight ~27 kDa.
Flow cytometry testing of fixed and permeabilized human HL60 cells with SIP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIP antibody.

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NSJ-R30852-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SIP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CACYBP (Calcyclin-binding protein), also called SIP (SIAH Interacting Protein), is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human SIAH Interacting Protein (NTRWDYLTQVEKECKE) was used as the immunogen for this SIP antibody (100% homologous in human, mouse and rat).
Limitation:
This SIP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9HB71