Green fluorescent protein (GFP) was first isolated by Dr. Osamu Shimomura in 1961. Since then, fluorescent protein (FP) technology has made drastic advancements by many researchers. Currently, FP technology is the most popular tool for visualizing target proteins. Dr. Shimomura, Martin Chalfie and Roger Y. Tsien won the 2008 Nobel Prize in Chemistry to mark their great achievements in life science research.
MBLI offers the CoralHue® FP series such as Keima-Red, Kusabira-Orange, and many others. We also offer antibodies against FPs.
FP antibodies are used for multiple purposes including pull-down assays, used as epitope-tag, increasing fluorescent intensity if the FP fluorescence is not bright enough, as well as other applications. MBLI offers polyclonal and monoclonal anti-GFP and anti-RFP antibodies with various conjugates including HRP, Agarose, and Magnetic beads. In fact, many researchers prefer to use our bead conjugates for pull-down assays. Our GFP polyclonal antibody (598) is highly regarded to have high performance and reliability among many reseachers. There are over 500 citations to date using this FP antibody.
| Code# | Description | Clone | Applications | Conjugates Available | Notes |
|---|---|---|---|---|---|
| 598 (sample sizes available) | Anti-GFP pAb | Polyclonal | WB, IP, IC, IH, ChIP*, Immunoelectron Microscopy | HRP, HRP-DirecT | Cumulative citations over 500 |
| D153 (sample sizes available) | Anti-GFP mAb | RQ2 | IP, IC | Agaraose, Magnetic beads, Magnetic Agarose, Alexa-Fluor | Excellent performance in IP and staining |
| PM005 (sample size available) | Anti-RFP pAb | Polyclonal | WB, IC, IH* | HRP, HRP-DirecT | Highly cited |
| M165 (sample size available) | Anti-RFP mAb | 3G5 | IP, FCM, IC | Agarose, Magentic beads, Magentic Agarose | Specialized for IP |
| M204 (sample size available) | Anti-RFP mAb | 1G9 | WB | HRP, HRP-DirecT | Specialized for WB |
| M208-3 (sample size available) | Anti-RFP mAb | Cocktail (1G9 and 3G5) | WB, IP, FCM, IC | For multiple application |
There are many FP variants available today from different resources. Are there the same number of antibodies as FP variants? The answer is no, because FP antibodies have the possibility to cross react to various variants due to high homology among those variants. Our antibodies have been tested for cross reactivity to some variants as shown in the *table below. An open circle shows we have tested our FP antibody with the corresponding variant. N.T. indicates that we have not tested against the variant.
Please don’t fret if you don’t see your variant listed! If your FP is not seen in the table above, we can estimate cross reactivity by running a homology search between our antigen and your FP. The following link is a protein BLAST search between our GFP antigen (full length of GFP derived from Aequorea victoria) and your FP. If the homology is high enough, polyclonal antibody (598) has a good possibility that it will cross react with your FP because this antibody has multiple epitopes.
MBL products are distributed In the UK and Ireland by Caltag Medsystems. If you have any questions about the products in this blog, please contact us.