KACTUS have developed a dsRNA Quick-Quantification Kit to detect the presence of undesired dsRNA molecules in IVT reactions or dsRNA in viral/non-viral nucleic acid extracts.
Product Specificiations:
- Detection Range: 0.078ng/µL~5ng/µL
- Sensitivity: 0.078ng/µL
Product Description:
MDA5 dsRNA Quick-Quantification Kit is used to detect the presence of undesired dsRNA molecules in IVT reactions or dsRNA in viral/non-viral nucleic acid extracts. MDA5 is an innate immune receptor for double-stranded RNA (dsRNA) with dsRNA-dependent ATPase activity. Single-stranded RNA (ssRNA) doesn’t stimulate MDA5 ATPase activity, which enables high specificity for dsRNA detection. Upon dsRNA binding, MDA5 ATPase is activated, converting ATP into ADP+Pi. Then, inorganic phosphate (Pi) reacts with malachite green reagent and forms coloured compounds. Colourimetric absorption at 620nm is proportional to dsRNA concentration.
Caltag Medsystems is the distributor of KACTUS products in the UK and Ireland. If you have any questions about these products, please contact us.