KACTUS: MHC Monomer & Tetramer Product Validation Data
KACTUS has demonstrated, via ELISA and SPR binding assays, that their MHC-peptide complexes and loaded peptide-ready MHCs have good binding affinity to TCRs. Their MHC products are well-suited for applications such as antibody binding, affinity research, TCR screening, and blocking assays.
Figure 1. Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR at 2µg/ml (100ul/well) on the plate can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag with an EC50 of 26.6ng/ml determined by ELISA.
Figure 2. Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Monomer, His Tag with the EC50 of 31.3ng/ml determined by ELISA.
Figure 3. Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer with an affinity constant of 4.62 nM as determined in SPR assay (Biacore T200).
Figure 4. In SPR analysis, the GP100 TCR & Anti-CD3 Bispecific Fusion can bind to the HLA-A*02:01&B2M&GP100 (YLEPGPVTA) complex tetramer, with an affinity constant of 0.196 nM.
Figure 5. Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.3991 µM as determined in SPR assay (Biacore T200).
Figure 6. Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Tetramer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.111 µM as determined in SPR assay (Biacore T200).
Figure 7. Flow Cytometry Analysis of OT1 Mouse Whole Blood Cells and T Cells Using PE-SA Assembled Tetramers. The right panel displays the binding of OT1 mouse T cells to the Mouse H-2K(b) & B2M & OVA (SIINFEKL) PE-SA assembled tetramer, with a significant shift in fluorescence and higher percentage of MHC PE-H positive cells (39.60% in Q5-4), compared to the left control, demonstrating effective binding and tetramer assembly.
Figure 8. Flow Cytometry Analysis of SIINFEKL Peptide-Pulsed OT1 Mouse Spleen Cells Using PE-SA Assembled Tetramers. The right panel displays the binding of OT1 mouse spleen cells to the custom-assembled tetramer (Mouse H-2K(b) & B2M & OVA (SIINFEKL) PE-SA), with a significant shift in fluorescence and higher percentage of MHC PE-H positive cells (97.41% in Q5-4), demonstrating effective binding and tetramer assembly.
Caltag Medsystems is the distributor of KACTUS products in the UK and Ireland. If you have any questions about these products, please contact us.
KACTUS: MHC Monomer & Tetramer Product Validation Data