abm has been innovating to set the next gold standard in reverse transcriptase performance. With improved thermostability, sensitivity, robustness, and resistance to contaminants, abm’s OneScript® brand of reverse transcriptases set a new benchmark for cDNA synthesis. Their OneScript® Hot is the only Reverse Transcriptase in the world that preserves performance at 50-80˚C, making it useful for even the most challenging projects. Engineered for both research or demanding diagnostic product applications, their premium enzymes will meet all your project needs in terms of quality and budget.
Products Available:
RT MasterMixes
Easily synthesize cDNA in a single step with abm’s All-In-One 5X RT MasterMix with gDNA Removal*. This optimised solution includes abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a perfectly balanced mix of Oligo (dT)s and Random Primers, all in ONE convenient tube!
For applications using pure RNA where genomic DNA removal is not required, try their OneScript® Hot 5X RT MasterMix. It offers a faster protocol for an even more efficient cDNA synthesis.
| PRODUCT | CAT. NO. |
|---|---|
| OneScript® Hot 5X RT MasterMix | ABM-G590 |
| All-In-One 5X RT MasterMix with gDNA Removal* | ABM-G592 |
Genomic DNA Removal*
Efficient cDNA Synthesis
Easy One-Step Setup
Super Simple
All-In-One 5X RT MasterMix with gDNA Removal is a convenient and ready-to-use formulation for first-strand cDNA synthesis with an easy one-step setup that reduces pipetting and sample handling. Simply add your RNA sample with our mastermix and incubate.
Accurate cDNA Synthesis
Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible.
OneScript® Hot Reverse Transcriptase
Synthesise cDNA from complex RNA templates with abm’s OneScript® Hot Reverse Transcriptase. Engineered for outstanding thermostability, their OneScript® Hot Reverse Transcriptase is ideal for handling secondary structures (e.g. GC-rich templates) as well as long, degraded, or contaminated RNA samples, making it the superior choice for challenging RNA templates.
| PRODUCT | CAT. NO. |
|---|---|
| All-In-One 5X RT MasterMix with gDNA Removal | ABM-G592 |
| OneScript® Hot 5X RT MasterMix | ABM-G590 |
| OneScript® Hot cDNA Synthesis Kit | ABM-G594 |
| OneScript® Hot Reverse Transcriptase | ABM-G593 |
| MegaFi™ One-Step RT-PCR | ABM-G597 |
Extreme thermostability At 50-80°C
Transcribes degraded samples and resists inhibitors/contaminants
High cDNA yields from difficult or ultra-low RNA samples
Outstanding Thermostability
OneScript® Hot Reverse Transcriptase has exceptional thermostability. OneScript® Hot Reverse Transcriptase (Cat. No. ABM-G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80˚C. The resulting synthesised cDNA was followed by PCR and visualised on a 1% agarose gel.
OneScript® Plus Reverse Transcriptase
Routine cDNA synthesis can be completed in just 10-15 minutes with abm’s OneScript® Plus Reverse Transcriptase! OneScript® Plus Reverse Transcriptase has improved thermostability and is engineered for high cDNA yields, high efficiency on low-input, long, or difficult RNA templates, and resistance to inhibitors and contaminants.
| PRODUCT | CAT. NO. |
|---|---|
| OneScript® Plus Reverse Transcriptase | ABM-G237 |
| OneScript® Plus cDNA Synthesis Kit | ABM-G236 |
| miRNA All-In-One cDNA Synthesis Kit | ABM-G898 |
High thermostability at 50-55°C
Synthesise 15 kb RNA Templates
10-15 minutes reaction time
Excellent Elongation Ability
OneScript® Plus Reverse Transcriptase can elongate RNA templates up to 15 kb in length. OneScript® Plus Reverse Transcriptase (Cat. No. ABM-G237) was used in a reaction with a range of human RNA fragments. The resulting synthesised cDNA was followed by PCR and visualised on a 1% agarose gel.
Unsure which reverse transcriptase is right for you?
| CHARACTERISTIC | OneScript® Hot | OneScript® Plus |
|---|---|---|
| Thermostability | 50-80°C | 50-55°C |
| Optimal Temperature | 60°C | 50°C |
| Maximum cDNA Elongation | 15 kb | 15 kb |
| Minimum Sample Input | 0.1 pg | 0.1 pg |
| Reaction Time | 10 min | 15 min |
| Inhibitor/Contamination Resistance | ••••• | ••• |
| Tolerance to Complex Templates | ••••• | ••• |
Information originally posted on: https://www.abmgood.com/Reverse-Transcriptase-PCR-cDNA-Synthesis.html
Caltag Medsystems is the distributor of abm products in the UK and Ireland. If you have any questions about these products, please contact us.