Transfection Reagents for In Vivo Delivery

Reagents based on Magnetofection^TM:

For General Transfection:

In Vivo DogtorMag^TM

In vivo DogtorMag™ Transfection Reagent has been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA, and oligonucleotides using magnetic nanoparticles coated with synthetic lipids (In vivo DogtorMag). DNA complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

In Vivo PolyMag^TM

In vivo PolyMag™ Transfection Reagent has been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA, and oligonucleotides using magnetic nanoparticles coated with specific polymers. DNA complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

For Infection:

In Vivo ViroMag

In vivo ViroMag Transfection Reagent has been designed for in vivo targeted transduction with any viral vector. Virus/nanoparticle complexes can be easily administrated through various injection routes such as systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular).

For Gene Silencing:

In Vivo SilenceMag^TM

In vivo SilenceMag™ is a rapid, simple and highly efficient method dedicated to transfecting small RNA (siRNA, miRNA) into target cells/tissue in vivo. It combines magnetic nanoparticles and small RNA that will be retained after injection at the magnetically targeted site. This targeted delivery method minimises systemic distribution, increases gene-targeted inactivation and reduces toxicity. Furthermore, the magnetic forces enhance the uptake of magnetic nanoparticles by the target tissue and thus improve the efficiency of silencing.

This allows for decreasing the required process time of delivery to a few minutes which is crucial for the improvement of in vivo small RNA delivery. In vivo SilenceMag™ is designed to meet in vivo grade quality.

In vivo SilenceMag™ benefits:

• Increased silencing efficiency.
• Targeted process (magnetically driven).
• Reduction of the systemic dissemination of siRNA/miRNA during injection.
• Reduction of the siRNA/miRNA doses.
• Work under non-permissive conditions (hypothermia, physiological flow conditions).
• Penetration of the siRNA/miRNA into tissues.
• Minimised toxicity.

Reagents based on Polyfection:

BrainFectIN™

BrainFectIN™ enables nucleic acid delivery into the central nervous system of small animals.

Major difficulties with gene delivery in the central nervous system are the weakness of standard non-viral gene carriers and the limitations associated with the use of viral particles (time-consuming and requires additional safety precautions). Unlike these methods, BrainFectIN™ is an original non-viral formulation that allows safe, easy and efficient nucleic acid delivery into the central nervous system of small animals.

This transfection reagent allows transfection of neural cells in specific brains following stereotaxic injection, with low immunogenicity and rapid and long-term transgene expression.

BrainFectIN™ has been designed by OZ Biosciences’ R&D team to meet in vivo grade quality (reagents performed under high manufacturing and quality standards and tested by strict quality controls).

BrainFectIN™ main advantages:

• Targeted process (stereotaxic injection).
• Good transfection efficiency.
• Reduction of the injection volume.
• Reduction of the DNA doses.
• Minimised toxicity.
• Low immunogenicity.
• Rapid and long-term transgene expression.

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Information provided by OZ Biosciences.

Caltag Medsystems is the distributor of OZ Biosciences products in the UK and Ireland. If you have any questions about these products, please contact us.