The understanding of protein-protein interactions is crucial for gaining insights into drug discovery and pathogenesis. It provides a unique glimpse into the natural communication between cells that is often unseen with in vitro assays. However, some attempts to visualise the interaction between two proteins can have some challenges. It can be time-consuming with cumbersome protocols often leading to unfavourable results accompanied with photobleaching.
MBLI’s technology, Fluoppi, simplifies the visualisation of protein-protein interaction. This innovative technology utilises fluorescent tags with a high signal-to-noise ratio, in turn requiring only a microscope to observe the fluorescence.
Fluoppi technology is a simple and effective way to obtain clear and precise results. To begin, create your constructs by conjugating Protein X with our FP-tag and Protein Y with our Ash-tag. From there, you can explore various conditions to determine the optimal fluorescence. Finally, test inhibitors or activators, so you can observe real-time protein-protein interaction events.
Fluoppi technology enables the researcher to easily identify and accurately measure the interaction between your Protein X and Y. Take your research to new heights with Fluoppi technology, which is invaluable for drug discovery, high throughput screening, and other applications.
| Product Code | Product Name |
| AM-VS0801M | Humanized Azami-Green for Fluoppi (phAG-MNL/MCL) |
| AM-VS0802M | Monti-Red for Fluoppi (pMonti-Red-MNL/MCL) |
| AM-8011M | Fluoppi Ver.2: Ash-hAG (Ash-MNL/MCL + hAG-MNL/MCL) |
REFERENCES:
- Koyano F, et al., Ubiquitin is phosphorylated by PINK1 to activate parkin. Nature. 510, 162-166 (2014).
Information provided by MBL.
Caltag Medsystems is the distributor of MBL products in the UK and Ireland. If you have any questions about these products, please contact us.