Abnova Corporation

Rat liver tissue lysate (non-denatured)

Product Code:
 
L038W2
Supplier:
 
Abnova Corporation
Host Type:
 
Rat
Regulatory Status:
 
RUO
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
 

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CodeSizePrice
L038W2-200ug200 ug£131.00
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This product comes from: Taiwan.
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  • Further Information
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Further Information

Antigen Target Species:
Rat
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method:
Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Product Description:
Whole tissue lysate (non-denatured)
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Recommended Dilutions:
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer:
In modified RIPA Lysis Buffer.
Tissue:
Liver

References

  • http://www.ncbi.nlm.nih.gov/pubmed/18363332