NSJ Bioreagents

Resistin Antibody / RETN

Product Code:
 
NSJ-V5621
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2, lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RETN/4325
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Resistin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human spleen tissue with Resistin antibody (clone RETN/4325). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot analysis of human heart tissue lysate using Resistin antibody (clone RETN/4325). Predicted molecular weight ~11 kDa.
3 / 3
SDS-PAGE analysis of purified, BSA-free Resistin antibody (clone RETN/4325) as confirmation of integrity and purity.

IHC staining of FFPE human spleen tissue with Resistin antibody (clone RETN/4325). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human heart tissue lysate using Resistin antibody (clone RETN/4325). Predicted molecular weight ~11 kDa.
SDS-PAGE analysis of purified, BSA-free Resistin antibody (clone RETN/4325) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V5621-20UG20 ug£264.00
Quantity:
NSJ-V5621-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Application Note:
Optimal dilution of the Resistin antibody should be determined by the researcher.
Description:
The cysteine-rich, adipose tissue-specific, secretory factor resistin (resistance to Insulin) also known as ADSF, is a secreted hormone that potentially links obesity to diabetes. Resistin is rich in serine and cysteine residues and contains a unique cysteine repeat motif. Resistin and the resistin-like molecules share the characteristic cysteine composition and other signature features. Resistin-like alpha is a secreted protein that has restricted tissue distribution and is most highly expressed in adipose tissue. Another family member, resistin-like beta, is a secreted protein expressed only in the gastrointestinal tract, particularly in the colon, in both mouse and human. Resistin-like beta expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant fragment of human RETN protein (within amino acids 19-108) was used as the immunogen for the Resistin antibody.
Limitation:
This Resistin antibody is available for research use only.
Localisation:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
Q9HD89