NSJ Bioreagents

ALK Antibody / Anaplastic Lymphoma Kinase

Product Code:
 
NSJ-V5625
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ALK/1503
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the Anaplastic Lymphoma Kinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing (A) recombinant protein and (B) human HepG2 cell lysate using Anaplastic Lymphoma Kinase antibody (clone ALK/1503). Predicted molecular weight ~180 kDa but may be observed at higher molecular weights due to glycosylation. Caspase 3 induced cleavage results in an ~60 kDa intracellular ALK fragment.
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Western blot testing (A) recombinant protein and (B) human HepG2 cell lysate using Anaplastic Lymphoma Kinase antibody (clone ALK/1503). Predicted molecular weight ~180 kDa but may be observed at higher molecular weights due to glycosylation. Caspase 3 induced cleavage results in an ~60 kDa intracellular ALK fragment.

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CodeSizePrice
NSJ-V5625-20UG20 ug£264.00
Quantity:
NSJ-V5625-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml
Application Note:
Optimal dilution of the Anaplastic Lymphoma Kinase antibody should be determined by the researcher.
Description:
The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human Anaplastic Lymphoma Kinase protein fragment (amino acids 1360-1460) was used as the immunogen for the Anaplastic Lymphoma Kinase antibody.
Limitation:
This Anaplastic Lymphoma Kinase antibody is available for research use only.
Localisation:
Cell membrane
Purity:
Protein A/G affinity
Uniprot #:
Q9UM73